I spent three days with fellow co-workers at the Golden LEAF Biomanufacturing Training & Education Center at NC State University; this time the course was geared towards downstream bioprocessing. I think Ken is totally tired of me talking about green fluorescent protein (GFP).
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Our course instructor was Dr. Gary Gilleskie, Teaching Associate Professor with BTEC (he is the tall one on the right).
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Jose homogenizing cell paste (the cell paste smelled way nasty - after all it was genetically modified
E. coli).
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Homogenizer samples under fluorescent light
(L to R: zero passes to five passes).
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Carl pipetting.
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Megan and I with GFP.
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Jose and Rebecca.
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Me cleaning a bench-top disc-stack centrifuge.
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The gang eating at the Cheesecake Factory.
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Lori, Anita, and me.
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Anita with a sample of GFP from the ultrafiltration step.
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A group of us ate at Crook's Corner in Chapel Hill - talk about delicious Southern cuisine! I had Green Peach Salad w/ mint & black pepper and their Carolina Sampler (North Carolina hickory smoked, pit cooked, pork barbecue served w/ hoppin' John, collard greens & black pepper cornbread).
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For dessert, I had the Buttermilk Pie - check out the menu description.
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The Buttermilk Pie!!! YUM!!!
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Capture of GFP by anion exchange chromatography.
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L to R: Clarified GFP Lysate, Flowthrough, and GFP Q Seph Eluate.
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Raleigh Durham Airport - ready to go home.
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