Tuesday, August 25, 2009

Training in North Carolina @ BTEC

I spent three days with fellow co-workers at the Golden LEAF Biomanufacturing Training & Education Center at NC State University; this time the course was geared towards downstream bioprocessing. I think Ken is totally tired of me talking about green fluorescent protein (GFP).


Our course instructor was Dr. Gary Gilleskie, Teaching Associate Professor with BTEC (he is the tall one on the right).


Jose homogenizing cell paste (the cell paste smelled way nasty - after all it was genetically modified E. coli).


Homogenizer samples under fluorescent light
(L to R: zero passes to five passes).


Carl pipetting.


Megan and I with GFP.


Jose and Rebecca.


Me cleaning a bench-top disc-stack centrifuge.


The gang eating at the Cheesecake Factory.






Lori, Anita, and me.


Anita with a sample of GFP from the ultrafiltration step.


A group of us ate at Crook's Corner in Chapel Hill - talk about delicious Southern cuisine! I had Green Peach Salad w/ mint & black pepper and their Carolina Sampler (North Carolina hickory smoked, pit cooked, pork barbecue served w/ hoppin' John, collard greens & black pepper cornbread).


For dessert, I had the Buttermilk Pie - check out the menu description.


The Buttermilk Pie!!! YUM!!!


Capture of GFP by anion exchange chromatography.


L to R: Clarified GFP Lysate, Flowthrough, and GFP Q Seph Eluate.


Raleigh Durham Airport - ready to go home.

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